A listing of the hazards associated with work in the laboratory infectious agents, toxins radioactive materials, etc. June 2010 page 1 of 185 institutional biosafety manual the purpose of this manual is to define the biological safety policies and procedures pertaining to research operations at boston university and boston medical center. A biosafety level bsl, or pathogenprotection level, is a set of biocontainment precautions required to isolate dangerous biological agents in an enclosed laboratory facility. Centres for disease control and prevention, national institute of health, u. The critical aspects of biosafety, biosecurity, and biocontainment have been in. Laboratory biosafety current staff university of queensland.
Who safe management of wastes from healthcare activities. All procedures are performed carefully to minimize the creation of splashes or aerosols. Access to the lab is more controlled than for bsl 1 facilities. Who laboratory biosafety manual lbm revision update.
Useful dilutions of wescodyne and common household bleach. Institutional biological safety manual 2010 table of contents. The institutional biosafety officer is responsible for ensuring that laboratories are working safely. Using toxichazardous substancesinfectious agentsrecombinant dna 27 c. Who laboratory biosafety manual, 3rd pdf icon external icon who laboratory biosafety guidance related to the novel coronavirus 2019ncov world health organization pdf icon external icon cdc 2007 guideline for isolation precautions. The manual now covers risk assessment and safe use of recombinant dna technology, and provides guidelines for the comissioning and. World health organization laboratory biosafety manual third edition. Biosafety in microbiological and biomedical laboratories bmbl quickly became the cornerstone of biosafety practice and policy in the united states upon first publication in 1984. Biosafety manual may 2010 page 2 class i biosafety cabinet an enclosure with an inward airflow through the front opening. Footnote cen workshop 31 laboratory biosafety and biosecurity. Biosafety manual laboratory manual that gives practical guidance on overall laboratory operation to provide a safe environment for the use of biological materialsrecombinant dna.
Requirements, responsibilities and guidelines for the safe handling and containment of microorganisms are set out in asnzs 2243. Responsibilities bso, ehs, ibc, pi, lab personnel training. Provides protection for the worker and the laboratory environment but not to product being utilized in the cabinet. For this new edition, therefore, the manual has been. The authors and editor of this revised second edition continue the nowestablished who tradition of. Who consultative meeting on highmaximum containment. The laboratory biosafety guidelines were initially developed to guide government, industry, university, hospital, and other public health and microbiological laboratories in their development of biosafety policies and programs. Basic, containment and maximum containment bsl yet to be defined technology. Agent summary statements for some agricultural pathogens. The names and contact information of the principal investigator, laboratory supervisor and or. This manual provides updated, standardized, evidencebased procedures and recommendations for laboratory managers, scientists and technicians to follow in examining human semen.
This manual provides updated, standardized, evidencebased procedures and recommendations for laboratory managers, scientists and technicians to follow in examining human. A laboratory specific biosafety manual must be prepared and adopted as policy. University of toledo biosafety and security manual page 2 conducting research at biosafety level bmbl, cdcnih guidelines. Please refer to the following links for further details. World health organization, 2010 diagnosis of malaria. Standard for managing biological risk in the veterinary laboratory and animal facilities provides a biorisk analysis and biorisk management approach. Biosafety level 1 bsl1 is the basic level of protection and is.
Wcln resources wisconsin state laboratory of hygiene. Presentation overview who biosafety manual key concepts evidence and riskbased approach way forward 3. In this video, youll gain an understanding of the different containment levels. For more than 20 years, since it was first published in 1983, the laboratory biosafety manual has provided practical guidance on biosafety techniques for use in laboratories at all levels.
After attending this session, participants will be able to differentiate the 3rd and 4th edition of the who laboratory biosafety manual. Research safety and training manual veterans affairs. Biosafety in microbiological and biochemical laboratories, 4th ed. The laboratory s written biosafety manual details any needed immunizations e. Biosafety and biosecurity as essential pillars of international health. Whoeurope organized brm training in tajikistan 20 and turkmenistan 2014. Aug 06, 2010 laboratory biosafety manual third edition world health organization geneva 2004 slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. We promote biological safety at the nih through a broad range of. Tuberculosis laboratory biosafety manual v executive summary following a technical consultation held between the world health organization who and the united states centers for disease control and prevention cdc in atlanta, ga, in september 2008 on strategies, approaches and partnerships that could be implemented to improve laboratory biosafety. The guidelines also serve as a technical document providing information and recommendations on the. Who laboratory manual for the examination of human semen and spermcervical mucus interaction. Biosafety manual biosafety manual for the university of nevada, reno. Each of the 98 subcategories addresses a specific laboratory practice that is relevant to the individual category.
Biosafety manual ibcapproved version may 18, 2010 printed copies are not official versions of this manual. The laboratory supervisor must ensure that laboratory personnel demonstrate proficiency in standard and special microbiological practices before working with bsl2 agents. The laboratorys written biosafety manual details any needed immunizations e. The canadian biosafety handbook cbh, 2 nd edition, 2016 is intended as a companion document to the canadian biosafety standard cbs, 2 nd edition, 2015. Department of health and human cch laboratory safety manual. Who laboratory manual for the examination and processing. Though the 4th edition is in draft form the time to. The international cartagena protocol on biosafety deals primarily with the agricultural definition but many advocacy groups seek to expand it to include postgenetic threats. Who laboratory biosafety manual lbm lbm has served the global biosafety community for more than 30 years with practical guidance on biosafety who manual 1st edition, 1983 risk group. Who laboratory biosafety manual updates absa conference. This includes a fire extinguisher, fire blanket, eyewash station, safety shower and gas cutoff. Mssm biosafety manual 2nd edition 4 2010 table of contents c. Class ii biosafety cabinet an enclosure with an inward airflow through the front opening. Biosafety manual the princeton university biosafety manual is intended to be a resource for information, guidelines, policies, and procedures that will enable and encourage those working in the laboratory environment to work safely and reduce or eliminate the potential for exposure to biological hazards.
Dr kazunobu kojima who headquarters, geneva who laboratory biosafety manual revision update laboratory design monograph working group meeting oxford, united kingdom 0708 february 2019 2. Guidelines for safe work practices in human and animal medical diagnostic laboratories cdc cdc prevention guidelines database. Who laboratory manual for the examination and processing of human semen 5th ed. Laboratory biosafety manual world health organization. Definitions risk groups, biosafety level risk assessment. Standard for managing biological risk in the veterinary laboratory and animal facilities. During this course you will be working with microorganisms that are classifed as biosafety level i and biosafety level ii organisms.
A tool for assessment of animal health laboratory safety. United states centers for disease control and prevention, which contains. Cdcaphl laboratory biosafety competencies for the bsl2, bsl. Nonserial publication reprint under study world health organization. Basic malaria united states centers for disease control and. In tajikistan, the brm training supported a european union and. The biosafety manual must be available and accessible.
Oecd series on principles of good laboratory practice and compliance monitoring, number 1. The uw biosafety manual provides students, staff, and faculty at uw with information that is necessary to protect them and the surrounding community from possible hazards associated with the use of biohazardous agents and recombinant or synthetic dnarna recdna molecules all laboratories and facilities using biohazards should have an electronic or paper copy of the current biosafety manual. The third edition of the who laboratory biosafety manual is a helpful reference and guide to nations that accept the challenge to develop and establish national codes of practice for securing microbiological assets, yet ensuring their availability for clinical, research and epidemiological purposes. Chapter 1620 canadian biosafety handbook, second edition. Laboratory biosafety manual university of west florida. Who guidance on regulations for the transport of infectious substances 20072008. Major revisions to the 2014 institutional laboratory. Biosafety publications biorisk management laboratory biosafety manual third edition.
In addition, you should be aware of all exits from the rooom. Each individual laboratory must supplement this manual with laboratory specific policies. The who laboratory biosafety manual 4th edition youtube. Laboratory biosafety manual third edition for more than 20 years, since it was first published in 1983, the laboratory biosafety manual has provided practical guidance on biosafety techniques for use in laboratories at all levels. The work areas should be kept clean and dust free to prevent contamination of samples and laboratory acquired infections. As a former member of the world health organizations biosafety advisory group, she was also a key figure in the development of the whos laboratory biosafety manual. Who laboratory manual for the examination and processing of.
Appendix c laboratory biosafety level 1 and 2 criteria. Tuberculosis laboratory biosafety manual laboratory biorisk management. If you continue browsing the site, you agree to the use of cookies on this website. The biosafety policy and the biosafety program at ohio university ou were established to prevent infection, promote safety in research studies, ensure safe handling of biological agents, provide for safe disposal of infectious laboratory wastes. As a followup to the november 30 biosafety presentation, a rudimentary, workinprogress version of the laboratory biosafety manual has been posted on the research integrity and compliance website. Good microbiological technique and appropriate use of biosafety equipment by well trained staff remain the fundamental elements of laboratory biosafety.
The levels of containment range from the lowest biosafety level 1 bsl1 to the highest at level 4 bsl4. At ryerson university, safety means that lab personnel are protected from exposures to infectious agents. Footnote 5 it is a guidance document for canadian facilities where human and terrestrial animal pathogens are handled, that updates the guidelines originally published as part ii of the. Oecd principles in good laboratory practice as revised in 1997.
Who 2010 responsible life sciences research for global health security. For more than 20 years, since it was first published in 1983, the laboratory biosafety manual has provided practical guidance on biosafety techniques for use in. Before using the printed copy, verify that it is the most current version. A manual of written standard operating procedures sops for the laboratory, in combination with this manual and the cdcnih publications biosafety in microbiological and biomedical laboratories, 5th edition and guidelines for research involving recombinant or synthetic nucleic acid molecules, provide general requirements for working with biological agents. Historically, the information in this publication has been advisory is nature even though legislation and regulation, in some circumstances, have overtaken it and made compliance with the guidance provided mandatory. She currently serves as a member of the who tb global laboratory initiative consultants roster for tb biosafety. Who guidance on regulations for the transport of infectious substances 2009 2010 publication. Laboratory safety manual touro university california. For this new edition, the manual has been extensively revised and expanded. Michigan technological university laboratory biosafety manual. Who instructions for storage and transport of suspected or confirmed human and animal specimens and virus isolates of pandemic h1n1 2009. The world health organization who laboratory biosafety.
Cohen, centers for disease control and prevention, atlanta, ga, usa. Biosafety in the mycobacteriology laboratory stop tb partnership. Chapter 4 and appendix 9 of the laboratory biosafety manual for specific information on sops. Revisions on terrestrial manual chapters in 2015 conceptual changes chapter 1. Who laboratory biosafety manual having served for global biosafety community hereinafter, who manual for more than 20 30 years, since it was first published in 1983, the laboratory biosafety manual has provided practical guidance on biosafety techniques for use in laboratories at all levels. The development of this fourth edition of the laboratory biosafety manual has. Environment directorate, organisation for economic cooperation and development, paris, france. The manual encouraged countries to accept and implement basic concepts in biological safety and to. Biosafety records records that are retained as required by regulatory and institutional policies for. The terminology for the 4 lmts areas and 20 categories is consistent with that found in the world organisation for animal health oie chapter biosafety and biosecurity.
This biosafety manual has been developed to provide general policies and procedures when working with biological agents at the university of nevada, reno. Who has published its interim guidance for laboratory biosafety related to 2019ncovcovid19sarscov2. Performing a risk assessment laboratory biosafety manual, 3rd edition, 2004 link to world health organization website guidelines for safe work practices in human and animal medical diagnostic laboratories link to cdc website. Preventing transmission of infectious agents in healthcare settings pdf icon.
Canadian biosafety handbook, second edition canada. Manual, the us biosafety in microbiological and biomedical laboratories, and the international consensus document cen workshop agreement cwa 15793. As needed, an adhoc committee may be formed to consider policy implications for the university of emerging technologies and processes that have a biosafety component. This biosafety manual represents the institutional practices and procedures for the safe use and handling of biological materials, recombinant dna and synthetic nucleic acids at stanford university. Laboratory specific biosafety manual checklist each laboratory is required to have a labspecific biosafety manual and cannot be borrowed or copied from another lab. Guidance documents manuals ebsa european biosafety. The new publication will combine the laboratory biosafety manual, 2nd ed. Laboratory biosafety manual were published in 1993 and 2004 respectively 2,3. To reflect these realities, the revised who laboratory biosafety manual laboratory biosafety manual will emphasize the use of practical measures to mitigate risks, including thorough risk assessments and evidencebased approaches to biosafety, rather than reliance. Laboratory specific biosafety manual checklist each laboratory is required to have a lab specific biosafety manual and cannot be borrowed or copied from another lab. Guidelines for biosafety laboratory competency cdcaphl pdf icon external icon. The who has issued a request for feedback on the new fourth edition of the who laboratory biosafety manual lbm. Semen analysis may be useful in both clinical and research settings, for investigating male fertility status as well as monitoring spermatogenesis during and following male fertility regulation and other interventions. Laboratory manual for the examirriltion of human semcn and sperm cervical mucus interaction.
Biosafety in microbiological and biomedical laboratories. Cen workshop 31 laboratory biosafety and biosecurity. Guide to the safe handling of radioactive materials in research. Emmett barkley, howard hughes medical institute, chevy chase, md, usa dr murray l. Biosafety in microbiological and biomedical laboratories bmbl. Laboratory biosafety and biosecurity risk assessment. In the united states, the centers for disease control and prevention cdc have specified these levels. Laboratory biorisk management for laboratories handling human. Sop 900 approval process for inactivation methods for infectious material. It is through their absolute commitment to quality, technical accuracy, and dedication to the professional. Biosafety manual office of environmental health and safety. The development of this third edition of the laboratory biosafety manual has been made possible through the contributions of the following, whose expertise is gratefully acknowledged.
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